|
KMID : 1004820130140040240
|
|
Journal of Biomedical Research
2013 Volume.14 No. 4 p.240 ~ p.248
|
|
|
Anti-proliferative efficacy comparison of conjugated linoleic acid on human cancer cell lines
|
|
Park Sook-Jahr
Kim Boh-Hyun
Kim Hoon-Gu
Kim So-Young
Hah Cyrus Kim
Cho Yong-Un
Kim Gon-Sup
Ha Yeong-Lae
|
|
|
Abstract
|
|
|
|
The anti-proliferative efficacy of t,t-conjugated linoleic acid (t,t-CLA), c9,t11-CLA, and t10,c12-CLA was compared in several human cancer cell lines. Gastric NCI-N87, liver Hep3B, pancreas Capan-2, and lung NCI-H522 cancer cells were incubated with 50 μM CLA isomers over a period of 6 days. The t,t-CLA inhibited the growth of all cancer cell lines to different extents, but c9,t11-CLA and t10,c12-CLA inhibited or stimulated the growth of the cancer cell lines. NCI-N87 cells were the most sensitive to growth inhibition and apoptosis from all CLA isomers tested. In NCI-N87 cells, CLA isomers reduced the release of arachidonic acid (AA) via the inhibition of cytosolic phospholipase A2 (cPLA2) activity, consequently reducing the production of PGE2 through the inhibition of cyclooxygenase-2 (COX-2). The efficacies of CLA isomers were in the following order (from most to least effective): t,t-CLA, t10,c12-CLA and c9,t11-CLA. Overall, these results imply that the anti-proliferative efficacy of t,t-CLA on cancer cells, especially NCI-N87 cells, was greater than other CLA isomers due to its induction of apoptosis through the inhibition of cPLA2 and COX-2 activities.
|
|
|
KEYWORD
|
|
|
anti-proliferation, human cancer cells, human gastric NCI-N87 cancer cells, t, t- CLA, apoptosis, cytosolic phospholipase A₂
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|